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Efficient recovery of whole blood RNA - a comparison of commercial RNA extraction protocols for high-throughput applications in wildlife species

Doreen Schwochow12*, Laurel EK Serieys1, Robert K Wayne1 and Olaf Thalmann13

Author Affiliations

1 Department of Ecology and Evolutionary Biology, University of California Los Angeles, 2149 Terasaki Life Science Building, Los Angeles, 90095, USA

2 Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, BMC Husargatan 3, Uppsala, SE75124, Sweden

3 Division of Genetics and Physiology, Department of Biology, University of Turku, Iltäinen Pitkäkatu 4, Turku, 520014, Finland

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BMC Biotechnology 2012, 12:33  doi:10.1186/1472-6750-12-33

Published: 27 June 2012

Additional files

Additional file 1:

Supplementary Information. The accompanied pdf-file provides in depth information regarding the sample preparation for 454 sequencing, two additional tables and a figure that depicts the sampling set up [56-58].

Format: PDF Size: 1.8MB Download file

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