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Open Access Highly Accessed Research article

Development of a transient expression assay for detecting environmental oestrogens in zebrafish and medaka embryos

Okhyun Lee, Charles R Tyler* and Tetsuhiro Kudoh*

Author Affiliations

Biosciences, College of Life and Environmental Sciences, University of Exeter, Exeter, Devon, EX4 4PS, UK

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BMC Biotechnology 2012, 12:32  doi:10.1186/1472-6750-12-32

Published: 24 June 2012

Additional files

Additional file 1:

Figure S1 GFP expression at 4 dpf embryos in the transient expression assay. Injected embryos without chemical exposure (A) or exposed to the oestrogenic chemicals 17α-ethinyloestradiol (1000 ngEE2/L) (B), 17β-oestradiol (1000 ng E2/L) (C) and nonylphonol (10 μgNP/L) (D) for 4 days. Head with lateral (L) and ventral (V) views (i and ii) and trunk with lateral view (iii). The shape of the liver is outlined with a white line. No GFP expression was observed in the unexposed control (A). EE2 and E2 induced GFP expression in the heart (h), liver (li), neuromasts (n) and somite muscles (sm) (B,C). In NP exposed larvae, GFP expression was observed mainly in the muscle and heart (D).

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