Comprehensive comparison of OFP fluorescence using spectrofluorometry in Arabidopsis thaliana . Ten to twelve independent transgenic lines of Arabidopsis overexpressing each OFP gene construct were selected. Separate measurements on intact rosettes from each of three individual plants were taken for each transgenic line and the average fluorescence intensity at the peak excitation wavelength is shown. All fluorescent measurements were normalized to the wild type control ecotype (Columbia).
Mann et al. BMC Biotechnology 2012 12:17 doi:10.1186/1472-6750-12-17