Fluorescence measurements for tobacco overexpressing tdTomato and tdTomato-ER.(A) Fluorescence measured at the peak excitation wavelength (581 nm) for each transgenic line. (B) Broad fluorescent intensity measured between 555 nm and 605 nm for tdTomato (solid lines) and tdTomato-ER (dotted lines). Ten independent transgenic lines were selected for overexpression of each of the constructs (tdTomato and tdTomato-ER).Leaf samples (youngest fully expanded leaf) from each of three individual plants were measured for each transgenic line and the average fluorescence intensity is shown. All fluorescent measurements were normalized to the non-transgenic (Xanthi) control.
Mann et al. BMC Biotechnology 2012 12:17 doi:10.1186/1472-6750-12-17