Table 2

Regression analysis was performed on PCR and LAMP-BART data sets obtained using various DNA extractions performed on a titration series of Bt11 maize reference tissue (0.1 - 5%; nd - not determined)

Target

Amplification

Extraction

Slope

Lowest detectable

Amplification


Sequence

Technique

Technique

(R2)

copy No (0.1% GM)

Efficiency E (+/- SD)


CaMV-35 S-p

PCR

Promega Wizard

0.522

205

2.64 (0.46)


CaMV-35 S-p

PCR

Nucleon Phytopure

0.131

438

2.32 (0.08)


CaMV-35 S-p

PCR

CTAB (10-1)

0.495

70

2.47 (0.82)


CaMV-35 S-p

PCR

Lumora's Simplified

nd

nd

nd


NOS-t

PCR

Promega Wizard

0.657

205

2.16 (0.41)


NOS-t

PCR

Nucleon Phytopure

0.471

438

2.13 (0.18)


NOS-t

PCR

CTAB (10-1)

0.775

70

1.91 (0.19)


NOS-t

PCR

Lumora's Simplified

nd

nd

nd


CaMV-35 S-p

LAMP-BART

Promega Wizard

0.565

205

1.55 (0.22)


CaMV-35 S-p

LAMP-BART

Nucleon Phytopure

0.471

641

1.24 (0.13)


CaMV-35 S-p

LAMP-BART

CTAB (10-1)

0.247

350

1.1 (0.03)


CaMV-35 S-p

LAMP-BART

Lumora's Simplified

0.403

40

1.21 (0.07)


NOS-t

LAMP-BART

Promega Wizard

0.215

205

1.70 (0.73)


NOS-t

LAMP-BART

Nucleon Phytopure

0.196

438

1.31 (0.33)


NOS-t

LAMP-BART

CTAB (10-1)

0.740

350

1.15 (nd)


NOS-t

LAMP-BART

Lumora's Simplified

0.119

40

1.25 (0.07)


Kiddle et al. BMC Biotechnology 2012 12:15   doi:10.1186/1472-6750-12-15

Open Data