Western blot analysis of HA products harvested from leaf apoplastic space extracts.N. benthamiana plants that were transiently transformed with pTRAa-H5 were infiltrated with buffer, following which the buffer was collected by low-speed centrifugation. HA was detected in the apoplastic space extracts using rabbit anti-H5N1 antibody. Lane 1, protein ladder (Fermentas); lane 2, wild type plant control; lanes 3 – 6, H5 products of different pTRAa-H5-infiltrated leaf batches.
Mortimer et al. BMC Biotechnology 2012 12:14 doi:10.1186/1472-6750-12-14