Table 1

Kinetic constants of purified laccase from Bacillus species for ABTS, 2,6-DMP and SGZ oxidation.

Substrate


Laccases

ABTS

2, 6-DMP

SGZ


KM (μM)

kcat (s-1)

Assay conditions

KM (μM)

kcat (s-1)

Assay conditions

KM (μM)

kcat (s-1)

Assay conditions


B. pumilus

80 ± 4

291 ± 2.7

0.6 μM-10 mM, pH 4, RT

680 ± 27

11 ± 0.1

0.6 μM-10 mM, pH 7, RT

n.d.

*4.9

6 - 78 μM, <0.8% (v/v) DMSO pH 6.5, RT


n.d.

*66 ± 1.5

0.04 μM-1.25 mM (25% (v/v) DMSO), pH 7, RT


B. subtilis [26]

124 ± 17

322 ± 20

10 -200 μM pH 4, 37°C

216 ± 35

29 ± 4

10 -200 μM, pH 7, 37°C

18 ± 3

80 ± 4

1 -100 μM, pH 7, 37°C


B. licheniformis [25]

6.5 ± 0.2

83

5 -500 μM pH 4, RT

56.7 ± 1

28

5 -1000 μM, pH 7, RT

4.3 ± 0.2

100

1.25 -50 μM, pH 7, RT


Substrate ranges covered, pH and temperature are listed. n.m.: not measurable, due to substrate solubility. * The value was deduced from the highest observed rate.

Reiss et al. BMC Biotechnology 2011 11:9   doi:10.1186/1472-6750-11-9

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