Table 1 |
||||||
|
Purification of BmNPV-GFPuv-hPRR particles by Sephacryl S-1000 column chromatography |
||||||
|
Volume (ml) |
Titer (pfu ml-1) |
Virus (pfu) |
Protein (mg ml-1) |
Purity (pfu mg-1 protein) |
Recovery (%) |
|
|
|
||||||
|
Hemolymph |
7 |
2.38 × 108 |
1.67 × 109 |
37.3 |
6.38 × 106 (1) |
100 |
|
Sephacryl S-1000 chromatography |
30 |
1.67 × 107 |
4.86 × 108 |
0.011 |
1.52 × 109 (238) |
29 |
|
After ultracentrifugation |
||||||
|
Suspended with PBS |
0.5 |
2.97 × 107 |
1.49 × 107 |
0.111 |
2.68 × 108 (42) |
0.9 |
|
Suspended with 0.01% Triton X-100 |
0.5 |
3.33 × 108 |
1.66 × 108 |
0.110 |
3.03 × 109 (475) |
10 |
|
Suspended with 0.01% Tween 20 |
0.5 |
1.51 × 108 |
7.55 × 107 |
0.071 |
2.13 × 109 (334) |
4.5 |
|
Suspended with 0.01% PF-68 |
0.5 |
4.60 × 108 |
2.30 × 107 |
0.059 |
7.80 × 109 (1,222) |
1.4 |
|
|
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|
Kato et al. BMC Biotechnology 2011 11:60 doi:10.1186/1472-6750-11-60 |
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