Table 2

Amino acid sequences of zebrafish wild-type (WT) and mutated Aqp3b

Construct

Loop A

Loop B

Loop C

Loop D

Loop E


DrAqp3b-WT

HILSGGS

    HG
MFLTV

SGGHINP

    T
VTFSLCLLG
    RE
PW
    R
KFP

FATYPSK

    H
LTLL

IVDP

    Y
NNPIPQGLEA

AVNP

    A
RDLGPRIFTAIAG

H53A

HILSGGS

    A
GMFLTV

SGGHINPTVTFSLCLLGREPWRKFP

FATYPSKHLTLL

IVDPYNNPIPQGLEA

AVNPARDLGPRIFTAIAG

G54A

HILSGGSH

    A
MFLTV

SGGHINPTVTFSLCLLGREPWRKFP

FATYPSKHLTLL

IVDPYNNPIPQGLEA

AVNPARDLGPRIFTAIAG

H53A/G54H

HILSGGS

    AH
MFLTV

SGGHINPTVTFSLCLLGREPWRKFP

FATYPSKHLTLL

IVDPYNNPIPQGLEA

AVNPARDLGPRIFTAIAG

H53A/G54H/T85A

HILSGGS

    AH
MFLTV

SGGHINP

    A
VTFSLCLLGREPWRKFP

FATYPSKHLTLL

IVDPYNNPIPQGLEA

AVNPARDLGPRIFTAIAG

T85A

HILSGGSHGMFLTV

SGGHINP

    A
VTFSLCLLGREPWRKFP

FATYPSKHLTLL

IVDPYNNPIPQGLEA

AVNPARDLGPRIFTAIAG

R95A

HILSGGSHGMFLTV

SGGHINPTVTFSLCLLG

    A
EPWRKFP

FATYPSKHLTLL

IVDPYNNPIPQGLEA

AVNPARDLGPRIFTAIAG

E96A

HILSGGSHGMFLTV

SGGHINPTVTFSLCLLGR

    A
PWRKFP

FATYPSKHLTLL

IVDPYNNPIPQGLEA

AVNPARDLGPRIFTAIAG

R99A

HILSGGSHGMFLTV

SGGHINPTVTFSLCLLGREPW

    A
KFP

FATYPSKHLTLL

IVDPYNNPIPQGLEA

AVNPARDLGPRIFTAIAG

H154A

HILSGGSHGMFLTV

SGGHINPTVTFSLCLLGREPWRKFP

FATYPSK

    A
LTLL

IVDPYNNPIPQGLEA

AVNPARDLGPRIFTAIAG

G54H/H154A

HILSGGSH

    H
MFLTV

SGGHINPTVTFSLCLLGREPWRKFP

FATYPSK

    A
LTLL

IVDPYNNPIPQGLEA

AVNPARDLGPRIFTAIAG

H53A/H154A

HILSGGS

    A
GMFLTV

SGGHINPTVTFSLCLLGREPWRKFP

FATYPSK

    A
LTLL

IVDPYNNPIPQGLEA

AVNPARDLGPRIFTAIAG

H53A/G54H/H154A

HILSGGS

    AH
MFLTV

SGGHINPTVTFSLCLLGREPWRKFP

FATYPSK

    A
LTLL

IVDPYNNPIPQGLEA

AVNPARDLGPRIFTAIAG

A217T

HILSGGSHGMFLTV

SGGHINPTVTFSLCLLGREPWRKFP

FATYPSKHLTLL

IVDPYNNPIPQGLEA

AVNP

    T
RDLGPRIFTAIAG

T85A/A217T

HILSGGSHGMFLTV

SGGHINP

    A
VTFSLCLLGREPWRKFP

FATYPSKHLTLL

IVDPYNNPIPQGLEA

AVNP

    T
RDLGPRIFTAIAG

Y182A

HILSGGSHGMFLTV

SGGHINPTVTFSLCLLGREPWRKFP

FATYPSKHLTLL

IVDP

    A
NNPIPQGLEA

AVNPARDLGPRIFTAIAG


Amino acid sequence of the five loops of WT and DrAqp3b mutants. The residues mutated and the substitutions are underlined in bold.

Chauvign√© et al. BMC Biotechnology 2011 11:34   doi:10.1186/1472-6750-11-34

Open Data