Changes in cell volume of oocytes expressing DrAqp3b-WT, DrAqp3b mutants or HsAQP3 in hypertonic solutions. (A) Oocytes expressing 1 ng of DrAqp3b-WT, -T85A, -H53A/G54H/T85A or HsAQP3 were exposed to 0.9 M sucrose in MBS for 10 min. (B-C) Oocytes expressing 1 (B) or 20 (C) ng of the same constructs were exposed to 1.3 M ethylene glycol in MBS for 10 min. Data from all panels are means ± SEM of 15-24 oocytes from 3-4 different batches.
Chauvigné et al. BMC Biotechnology 2011 11:34 doi:10.1186/1472-6750-11-34