Proteins isolated from both strains give virtually identical CD spectra. CD spectra of the multimeric proteasome complex PrcA-B (A), the dodecameric acyl-CoA carboxylase AccD5-AccA3 complex (B), CFP10-ESAT6 heterodimer (C), and monomeric protein ACPS (D) expressed in M. smegmatis mc2155 (WT = black) and M. smegmatis groEL1ΔC (grey) are virtually identical. For A and B, a concentration between 170 and 200 nM was used while for C and D, concentrations were between 5 and 10 μM.
Noens et al. BMC Biotechnology 2011 11:27 doi:10.1186/1472-6750-11-27