Table 1

Oligonucleotides employed in this study

Oligonucleotide

Size

Purpose


Number

Sequence

(nt)


Ml-08-5104

5'-TCGAGAAAAG ACATCATCAT CATCATCATA ACCAGGAGCA GGTGTCCCCA CTTACCCTCC TCGCTGGAGG TGGAGGGTAC-3'

80

XhoI-KpnI sense strand for XL1-HSA


ML-08-5105

5'-CCTCCACCTC CAGCGAGGAG GGTAAGTGGG GACACCTGCT CCTGGTTATG ATGATGATGA TGATGTCTTT TC-3'

72

XhoI-KpnI antisense strand for XL1-HSA


ML-08-5106

5- TCGAGAAAAG ACATCATCAT CATCATCATG ATCAGATGAT GCTGCCATGG CCAGCTGTGA CCCTGGGAGG CGGAGGGTAC -3'

80

XhoI-KpnI sense strand for XL2-HSA


ML-08-5107

5- CCTCCGCCTC CCAGGGTCAC AGCTGGCCAT GGCAGCATCA TCTGATCATG ATGATGATGA TGATGTCTTT TC-3'

72

XhoI-KpnI antisense strand for XL2-HSA


ML-08-5108

5'-TCGAGAAAAG ACATCATCAT CATCATCATT GGCAGCATAA AATCGATCTG CCATACAATG GTGCAGGAGG CGGAGGGTAC-3'

80

XhoI-KpnI sense strand for XL3-HSA


ML-08-5109

5'- CCTCCGCCTC CTGCACCATT GTATGGCAGA TCGATTTTAT GCTGCCAATG ATGATGATGA TGATGTCTTT TC-3'

72

XhoI-KpnI antisense strand for XL3-HSA


ML-08-5116

5'- TCGAGAAAAG AAACCAGGAG CAGGTGTCCC CACTTACCCT CCTCGCTGGA GGTGGAGGGT AC -3'

62

XhoI-KpnI sense strand for XL4-HSA


ML-08-5117

5'- CCTCCACCTC CAGCGAGGAG GGTAAGTGGG GACACCTGCT CCTGGTTTCT TTTC-3'.

54

XhoI-KpnI antisense strand for XL4-HSA


ML-08-5118

5'- TCGAGAAAAG AGATCAGATG ATGCTGCCAT GGCCAGCTGT GACCCTGGGA GGCGGAGGGT AC -3'

62

XhoI-KpnI sense strand for XL5-HSA


ML-08-5119

5'- CCTCCGCCTC CCAGGGTCAC AGCTGGCCAT GGCAGCATCA TCTGATCTCT TTTC -3'

54

XhoI-KpnI antisense strand for XL5-HSA


ML-08-5120

5'-TCGAGAAAAG ATGGCAGCAT AAAATCGATC TGCCATACAA TGGTGCAGGA GGCGGAGGGT AC-3'

62

XhoI-KpnI sense strand for XL6-HSA


ML-08-5121

5'-CCTCCGCCTC CTGCACCATT GTATGGCAGA TCGATTTTAT GCTGCCATCT TTTC-3'

54

XhoI-KpnI antisense strand for XL6-HSA


ML-09-3874

5'-ACGTGTCGAGA AAAGACATCA TCATCATCAT CATAACCAGG AGCAGGTGTC CCCAC-3'

45

Upstream primer for PCR to assemble H6α2AP(13-42)-HSA


ML 17225

5'-ACGTGGTACCG ACTCCTGGGG GACTCTTCAG-3'

30

Downstream primer for PCR to assemble H6α2AP(13-42)-HSA


Sheffield and Eltringham-Smith BMC Biotechnology 2011 11:127   doi:10.1186/1472-6750-11-127

Open Data