TEM images of αS-EGFP aggregates grown in potentially denaturing conditions. The scale bar is 200 nm wide and all images are shown at the same magnification. All samples were incubated for three weeks, and unless otherwise specified, the incubation temperatures were 37°C. The protein concentrations and other solution conditions are as follows: (A-B) 50 μM αS-EGFP at pH 2.4. (C) 50 μM αS-EGFP at pH 2.4 with 150 mM NaCl. (D-E) 50 μM αS-EGFP in pH 7.5 NaPhos with 15% TFE. (F) 50 μM αS-EGFP in pH 7.5 NaPhos with 10% TFE, after incubation at 22°C under quiescent conditions. (G) Same as F, but the solution contained 75 μM αS-EGFP. (H) Same as F, but the solution contained 15% TFE. (I) 50 μM αS-EGFP at pH 2.4 with 15% TFE. (J) 50 μM αS-EGFP at pH 2.4 with 15% TFE and 150 mM NaCl.
Anderson and Webb BMC Biotechnology 2011 11:125 doi:10.1186/1472-6750-11-125