TEM images of aggregates grown from seeded or unfiltered αS-EGFP stocks. The scale bar is 200 nm wide and all images are shown at the same magnification. Unless otherwise specified, all solutions contained PBS buffer (10 mM pH 7.5 NaPhos, 150 mM NaCl) and the samples were incubated with shaking at 37°C. The protein concentrations, incubation times t, and other solution conditions are as follows: (A-B) 75 μM unfiltered αS-EGFP, t = 3 weeks. (C) 150 μM unfiltered αS-EGFP, t = 3 weeks. (D) 35 μM unfiltered αS-EGFP in pH 7.4 Tris buffer with 100 mM NaCl, t = 3 weeks. (E-G) 30 μM filtered αS-EGFP plus ~4 μM unfiltered αS-EGFP "seed" in pH 7.4 Tris with 100 mM NaCl, t = 4 weeks. (H) 20 μM unfiltered αS-EGFP in pH 7.4 Tris with 100 mM NaCl, t = 4 weeks. (I-J) 150 μM filtered αS-EGFP plus ~ 8 μM unfiltered αS-EGFP "seed", t = 4 weeks.
Anderson and Webb BMC Biotechnology 2011 11:125 doi:10.1186/1472-6750-11-125