Figure 1.

TEM images of aggregates grown from Aβ40 labelled with three extrinsic fluorescent dyes. Each column corresponds to one fluorophore. The scale bar is 200 nm wide and all images are shown at the same magnification. Unless otherwise noted, the samples were incubated at 22°C under quiescent conditions. The protein concentrations, incubation times t, and other solution conditions are as follows: (A) 200 μM Hilyte Fluor 488-Aβ40 in water, t = 7 days. (B) 15 μM Hilyte Fluor 488-Aβ40 in 100 mM pH 7 NaPhos buffer, t = 8 weeks. (C) 10 μM Hilyte Fluor 488-Aβ40 in 50 mM pH 6 NaPhos, t = 4 weeks. (D) 10 μM Hilyte Fluor 488-Aβ40 in 50 mM, pH 7 NaPhos with 5% TFE, t = 10 weeks. (E) 25 μM TAMRA-Aβ40 incubated at 37°C with shaking in 50 mM pH 7 NaPhos with 10% TFE, t = 2 weeks. (F) 50 μM TAMRA-Aβ40 in 100 mM pH 7 NaPhos, t = 8 weeks. (G) 73 μM TAMRA-Aβ40 in 50 mM pH 7 NaPhos, t = 15 weeks. (H) 50 μM TAMRA-Aβ40 in 50 mM pH 6 NaPhos, t = 20 weeks. (I) 25 μM AMCA-Aβ40 incubated at 37°C with shaking in 50 mM pH 7 NaPhos containing 10% TFE, t = 2 weeks. (J) 25 μM AMCA-Aβ40, shaken overnight at 37°C, followed by incubation at 22°C under quiescent conditions for 5 weeks. (K) 20 μM AMCA-Aβ40 in 50 mM, pH 7 NaPhos with 100 mM NaCl, t = 4 weeks. (L) 20 μM AMCA-Aβ40 in 50 mM, pH 7 NaPhos, t = 4 weeks.

Anderson and Webb BMC Biotechnology 2011 11:125   doi:10.1186/1472-6750-11-125
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