Table 3

Parameters of NOX immobilization onto agarose via different chemistries.

Activated agarosea

Immobilization yield

Ψ (%)b

Expressed activity

Ae (%)c


Gx-aga

85

60


CNBr-aga

100

80


IDA-Cu2+-aga

100

80


a 6BCL agarose was activated with different functional groups as methods described in order to immobilized NOX through different chemistries, giving each one different properties to the final insoluble preparation. b Immobilization yield was calculated as follows; Ψ (%) = (Supernatant activity after incubation with the support/Supernantant activity before incubation with the support)*100.c Expressed activity was calculated; Ae (%) = (Activity/g of support)/(Immobilized activity/g of support)*100.

Rocha-Martín et al. BMC Biotechnology 2011 11:101   doi:10.1186/1472-6750-11-101

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