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Correction: Analysis of conditional gene deletion using probe based Real-Time PCR

Britta Weis1*, Joachim Schmidt2, Frank Lyko1 and Heinz G Linhart3

Author Affiliations

1 Division of Epigenetics, German Cancer Research Center, Heidelberg, Germany

2 Division of Translational Oncology, German Cancer Research Center, Heidelberg, Germany

3 Department of Gastroenterology and Hepatology, University Hospital Freiburg, Hugstetter Str. 49, 79106 Freiburg, Germany

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BMC Biotechnology 2010, 10:90  doi:10.1186/1472-6750-10-90

The electronic version of this article is the complete one and can be found online at:

Received:13 December 2010
Accepted:21 December 2010
Published:21 December 2010

© 2010 Weis et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Following publication of this article [1] the authors noticed that an incorrect probe reference was cited on page 3, 4, 5 and 6 ("UP #69, Roche Applied Science"). The correct probe that was used for the 1lox/2lox allele ratio analysis in the paper is as follows

Probe for 1lox/2lox allele quantification:

5'-6-FAM-atAaCtTCgtatagCATaCattatac-BHQ-1 -3'

(uppercase letters = LNA bases)

Manufacturer: EUROGENTEC, Seraing, Belgium

All other information and reaction conditions in the paper are correct as stated.



  1. Weis B, Schmidt J, Lyko F, Linhart HG: Analysis of conditional gene deletion using probe based Real-Time PCR.

    BMC Biotechnology 2010, 10:75. PubMed Abstract | BioMed Central Full Text | PubMed Central Full Text OpenURL