Comparison of the transfection efficacy of pmaxGFP with chemical reagents (Effectene, Lipofectamine 2000, Lipofectamine Plus, and PEI) and nucleofection. The vol/wt ratios (μl reagent/μg DNA) for Effectene and Lipofectamine 2000 transfection and the (+/-) charge ratios (PEI nitrogen residues/DNA phosphates) for PEI transfection are indicated in parentheses. Transfection efficacy is indicated by the black bar, and the relative number of adherent cells in the transfected cells was compared to the number in nontransfected control cultures is indicated by the white bar for (A) pig fetal fibroblast (P16), (B) primary pig tracheal epithelial (PTE) cells, and (C) primary human tracheal epithelial (HTE) cells. The error bars reflect the standard error of the mean (SEM), with n = 3.
Maurisse et al. BMC Biotechnology 2010 10:9 doi:10.1186/1472-6750-10-9