Specificity of RAbDMvII1 by Flow cytometry. Flow cytometric analysis of surface expressed EGFRvIII. A) 1 × 106 cells HC2 cells were stained with increasing concentration of RAbDMvIII: 0.1 μg (purple), 1 μg (red) and 2 μg (green). The dot blot (left panel) and histogram in blue represents human-IgG1 isotype control. B) NIH3T3 and HC2 cells were mixed in a 1:1 ratio prior to staining with RAbDMvIII revealing distinct identification of an EGFRvIII cell population. C) Overlay of flow cytometric plots of A431 (black) cells and HC2 cells (blue) stained with RAbDMvIII, showing clear selectivity for EGFRvIII expression over EGFR expression. D) Freshly dissociated human GBM tumor stained with RAbDMvIII shows the presence of surface EGFRvIII on 13% of cells (right panel). The dot blot (left panel) represents human-IgG1 isotype control.
Gupta et al. BMC Biotechnology 2010 10:72 doi:10.1186/1472-6750-10-72