Immunoprecipitation of EGFRvIII by RAbDMvIII. Immunoprecipitation was performed with RAbDMvIII, Monoclonal EGFRvIII antibody and RAbCD133 (negative control for our recombinant antibody) on HC2 and A431 cell lysates. A) HC2 lysates immunoprecipitated by Monoclonal EGFRvIII antibody, RAbDMvIII showed 145 kda band corresponding to EGFRvIII in the pellet whereas 145 kda band is seen in the sup of RAbCD133 when immunoblotted with E3138 antibody from sigma. B) On the other hand A431 cell lysate showed 170 kda protein band corresponding to EGFR in the supernatant of Monoclonal EGFRvIII antibody, RAbDMvII1 and RAbCD133samples.
Gupta et al. BMC Biotechnology 2010 10:72 doi:10.1186/1472-6750-10-72