Figure 7.

Structural alignment of the V domains from the 4B2A1, 7B2A1 and 2C TCRs. Alignments of the Vα (A) and Vβ (B) were made with MUSCLE [67] and annotated according to Hare et al. [68]. Amino acid positions mutated are indicated by colour code according to the source, orange[23] and green [24]), and the resulting residues are indicated at each position. 4B2A1 was originally misaligned regarding α chain residue 82. Ribbon representation of the 2C crystal structure (PDB ID: :1TCR) with the positions of the five mutations selected in the 4Vmut and 7Vmut variants, as given by the alignments in A and B (shown as spheres). The figure was prepared using PyMol (C).