Additional file 1:

Alignment of the 85 SNPs found in fragment AB of CytB of the fish family Scombridae. The first 49 SNPs are found in fragment A, while SNPs 50 - 85 are present in fragment B. Acronym of species as in Table 1 of manuscript. Forty-two tunas representing eight species of the Thunnus species complex Reference sequences: Thunnus thynnus [NCBI: NC_004901; portion 14652-14877 bp].

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Additional file 2:

Alignment of the 85 SNPs found in fragment AB of CytB of the fish family Scombridae. The first 49 SNPs are found in fragment A, while SNPs 50 - 85 are present in fragment B. Acronym of species as in Table 1 of manuscript. Twenty-eight mackerels (Scomber spp.). Reference sequence: Scomber japonicus [NCBI: AB018996]

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Additional file 3:

Alignment of the 85 SNPs found in fragment AB of CytB of the fish family Scombridae. The first 49 SNPs are found in fragment A, while SNPs 50 - 85 are present in fragment B. Acronym of species as in Table 1 of manuscript. Seventy-eight fish representing frigate tuna (Auxis thazard thazard), bullet tuna (Auxis rochei rochei), Atlantic bonito (Sarda sarda), little tunny (Euthynnus alletteratus), skipjack tuna (Katsuwonus pelamis). Reference sequence: Auxis thazard thazard [NCBI: DQ080314].

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Additional file 4:

Estimates of Net Evolutionary Divergence between Groups of Sequences. The number of base differences per sequence from estimation of net average between groups of sequences is shown. All results are based on the pairwise analysis of 151 sequences containing 85 variable positions. Standard error estimates are shown in the second column and were obtained by a bootstrap procedure (1000 replicates).

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