BMC Biotechnology

official impact factor 2.86

Open Access Research article

Moisture-induced solid state instabilities in α-chymotrypsin and their reduction through chemical glycosylation

Giselle M Flores-Fernández, Miraida Pagán, Mariangely Almenas, Ricardo J Solá and Kai Griebenow*

Author Affiliations

Department of Chemistry, University of Puerto Rico, Río Piedras Campus, P.O. Box 23346, San Juan, 00931-3346, Puerto Rico

For all author emails, please log on.

BMC Biotechnology 2010, 10:57 doi:10.1186/1472-6750-10-57

Published: 9 August 2010

Additional files

Additional file 1:

Water sorption isotherms of α-CT incubated at different RH at 50°C measured by Karl Fisher coulometry. BET water sorption isotherms of α-CT demonstrating water sorption equilibration after incubation at different RH

Format: TIFF Size: 384KB Download file

Open Data

Additional file 2:

SEC-HPLC chromatogram of α-CT incubated at 96% RH and 50°C. α-CT was dissolved at pH 5. Chromatograms depicts the monomer loss of α-CT after incubation at 96% RH

Format: TIFF Size: 2.7MB Download file

Open Data