Dissociation of chimeras in apricot callus. Explants were transformed initially with the binary vector pBin19-35SGusintron carrying the nptII transgene for kanamycin selection. The transgenic line was sub-cultured three times at monthly intervals and its DNA was used for real-time PCR experiments. Values are means from three biological replicates.
Faize et al. BMC Biotechnology 2010 10:53 doi:10.1186/1472-6750-10-53