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Open Access Highly Accessed Research article

Hydrocarbon phenotyping of algal species using pyrolysis-gas chromatography mass spectrometry

Dinesh K Barupal1, Tobias Kind1, Shankar L Kothari2, Do Yup Lee1 and Oliver Fiehn1*

Author Affiliations

1 Genome Center, University of California, Davis, USA

2 Department of Botany, University of Rajasthan, Jaipur, India

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BMC Biotechnology 2010, 10:40  doi:10.1186/1472-6750-10-40

Published: 21 May 2010

Additional files

Additional file 1:

Figure S1: Effect of temperature on the number of hydrocarbon related pyrolysis products in Botryococcus braunii. -C11H13 substructure shown as representative hydrocarbon.

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Additional file 2:

Table S1: Identification of pyrolysis products in Botryococcus braunii race B using GCMS and NISTMS Library 2005. Only deconovoluted components are shown here that are annotated as hydrocarbon substructure and that showed more than 70% similarity during NIST05 library searches.

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Additional file 3:

Table S2: Compounds annotations in pyGC-MS based on matching molecular masses and fragment spectra using the Dictionary of Natural Products database.

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Additional file 4:

Figure S2: Visualization of substructure annotation of using network graph in Cytoscape. Colored spheres are hydrocarbon related components. Colored triangles are non-hydrocarbon related components. Colors reflect retention time: blue = early elution; red = late elution. Yellow spheres were annotated by hydrocarbon substructure classifiers. Yellow triangles were annotated as non-hydrocarbon substructure classifiers.

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