Table 1 |
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|
Validation of Tm determinations for antiparallel duplex and parallel triplex formations |
||||||
|
Mean (°C) |
Standard Deviation |
Number |
Minimum (°C) |
Maximum (°C) |
CV% |
|
|
|
||||||
|
Antiparallel duplex |
||||||
|
Intra assay |
73.60 |
0.14 |
12 |
73.33 |
73.80 |
0.19 |
|
Inter assay |
73.65 |
0.09 |
6 |
73.52 |
73.80 |
0.12 |
|
Parallel triplex |
||||||
|
Intra assay |
75.03 |
0.08 |
12 |
74.89 |
75.13 |
0.11 |
|
Inter assay |
75.11 |
0.10 |
6 |
75.01 |
75.25 |
0.14 |
|
|
||||||
|
Intra- and inter-assay coefficient of variation for antiparallel duplex formation in sodium phosphate buffer pH 7.0 with 0.5 μM of each probe and parallel triplex formation in sodium acetate buffer pH 4.8 with 1.0 μM of each probe. |
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|
Schneider et al. BMC Biotechnology 2010 10:4 doi:10.1186/1472-6750-10-4 |
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