Figure 7.

Tm determined by UV absorbance compared with Tm on the LightCycler. Quadruplicate determinations for antiparallel duplex formation by UV absorbance (UV AD) as 1.0 μM of each probe and LightCycler (LC AD) in sodium phosphate buffer pH 7.0 as 0.5 μM of each probe and for parallel triplex formation by UV absorbance (UV PT) and LightCycler (LC PT) in sodium acetate buffer pH 5.0 with 1.0 μM of each probe.