Table 3 |
||||||
|
PCR screening results of transgenic insect cell lines. |
||||||
|
Construct |
Product 1 |
Product 2 |
Product 3 |
gag |
Neo |
Amp |
|
|
||||||
|
pXLNeoGag passage 15 in Sf21 cells |
+ |
+ |
+ |
+ |
+ |
+ |
|
|
||||||
|
pXLNeoGag passage 26 in Sf21 cells |
+ |
+ |
+ |
+ |
+ |
+ |
|
|
||||||
|
pXLNeoGag passage 23 in Sf9 cells |
X |
+ |
+ |
+ |
+ |
+ |
|
passage 7 |
+ |
|||||
|
passage 13 |
+ |
|||||
|
passage 93 |
+ |
|||||
|
|
||||||
|
pXLIE-SVGag passage 16 in Sf21 cells |
X |
+ |
X |
+ |
X |
+ |
|
|
||||||
|
pXlHr3ieGag passage 9 in Sf21 cells |
X |
+ |
X |
+ |
X |
+ |
|
|
||||||
|
pXLHSP70Gag passage 6 in Sf21 cells |
X |
+ |
X |
+ |
X |
+ |
|
|
||||||
|
Screening was carried out using primers complementary to different sections of the pXLBacIINeoGag vector. The presence of a PCR product is indicated with a plus (+) and absence with a cross (X). Confirmation of stable integration up to 93 passages was indicated by performing PCR amplification on several passages of pXLNeoGag in Sf9 cells (see bold font). |
||||||
|
Lynch et al. BMC Biotechnology 2010 10:30 doi:10.1186/1472-6750-10-30 |
||||||
