Table 3

List of forward and reverse primers used for semi-quantitative RT-PCR.

Name of Gene*

Forward primer

Reverse primer

LEA-5

5' ATGGCTCGCCCTTTCTCAAACG 3'

5' TCAGTTTTTCTTCAGAAGCATAGCTCTTAATTCGG 3'

Apx-1

5'ATGGCTAAGAACTATCCAAAAGTAAGC

GAAGAGTA3'

5' TTAGGCATCAGCAAATCCCAGCTCTG3'

Metallothionein

5' ATGTCTTGCTGCGGAGGAAACTG 3'

5' CACACGACAGGGTTTGAGAAGGTAC 3'

Profilin

5' ATGTCGTGGCAAACATACGTAGATGAGC 3'

5' CCAAGCCTTTCGACAATCATGTTGC 3'

Annexin

5' ATGGCTACCATTGTTGTTCCTGCC 3'

5' AGCTGTGTCTTGCTCCTTGTAGTGAG 3'

*LEA-5, late embryogenesis abundant protein-5; Apx-1, cytosolic ascorbate peroxidase -1.

Eswaran et al. BMC Biotechnology 2010 10:23   doi:10.1186/1472-6750-10-23