Research article
Yeast functional screen to identify genetic determinants capable of conferring abiotic stress tolerance in Jatropha curcas
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* Corresponding author: Sudhakar J Tangirala ts_johnson@relbio.com
1 Plant Metabolic Engineering Group, Reliance Life Sciences Pvt Ltd, Dhirubhai Ambani Life Sciences Center, R-282, Thane- Belapur Road, Rabale, Navi Mumbai- 400 701, India
2 DuPont Knowledge Centre, ICICI Knowledge Park, Genome Valley, Turkapalli, Shamirpet, Hyderabad 500 078, India
BMC Biotechnology 2010, 10:23 doi:10.1186/1472-6750-10-23
Published: 20 March 2010Additional files
Additional file 1:
Salinity treatment to seedlings. Twenty one-day-old J. curcas seedlings were treated with 0, 150 mM, 250 mM and 750 mM NaCl for 0 h, 2 h, 4 h and 8 h. Total RNA was extracted from root and leaf tissues of 150 mM NaCl treated seedlings at 0 h, 2 h and 8 h and semi- quantitative RT-PCR expression of selected J. curcas genes was performed.
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Additional file 2:
Process flow. Process flow of genetic screen: Outline of the process to identify and isolate specific genes from J. curcas involved in abiotic stress responses using yeast functional genetic screen.
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Additional file 3:
Genotype details. Strain details of genotype of yeast, Saccharomyces cerevisiae used in the screen.
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Additional file 4:
Stock composition. Stock compositions used in synthetic media
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Additional file 5:
Composition of synthetic selection media used in the functional screen. Composition of synthetic selection media used in the functional screen
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