Open Access Highly Accessed Research article

pEPito: a significantly improved non-viral episomal expression vector for mammalian cells

Rudolf Haase14, Orestis Argyros2, Suet-Ping Wong2, Richard P Harbottle2, Hans J Lipps3, Manfred Ogris4, Terese Magnusson4, Maria G Vizoso Pinto1, Jürgen Haas15 and Armin Baiker1*

Author Affiliations

1 Max von Pettenkofer-Institute, University of Munich, Munich, Germany

2 National Heart and Lung Institute, Imperial College London, London, UK

3 Institute for Cell Biology, University of Witten/Herdecke, Witten, Germany

4 Department of Pharmacy, University of Munich, Munich, Germany

5 Division of Pathway Medicine, University of Edinburgh, Edinburgh, UK

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BMC Biotechnology 2010, 10:20  doi:10.1186/1472-6750-10-20

Published: 15 March 2010

Additional files

Additional file 1:

Representative FACS-diagrams corresponding to figure 3A. This additional file depicts representative flow cytometry profiles corresponding to all bars shown in figure 3A (transiently transfected HEK293 cells).

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Additional file 2:

Representative FACS-diagrams corresponding to figure 4A.

Description: This additional file depicts representative flow cytometry profiles corresponding to all bars shown in figure 4A (stably selected mixed-clone HEK293 cells).

Format: PDF Size: 109KB Download file

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Additional file 3:

Quantification of expression profiles in vivo. This additional file depicts graphically the extended longitudinal expression study of the mice for up to 32 days. Luciferase expression is quantified using Xenogen Living Image software and represented as photons/sec/cm2/sr. Background level of light emission on non-treated animals is 1 × 106 photons/sec/cm2/sr. Standard error of the mean for each time point is indicated.

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