Detailed immunofluorescent detection of overexpressed tag-eGFP in vivo. Adjacent sections of the analyzed tissue described in Fig. 2 and 3 were immunofluorescently stained with anti-tag antibody using the dilutions given in table 2. A) A representative picture is shown for each tag/antibody combination, the left part shows eGFP fluorescence, the middle part shows the tag staining and the right part is a merged picture of the two individual pictures. Overlay pictures reveal the degree of anti-tag antibody sensitivity. Scale bar: 50 μm. B) Mean percentage transduced cells recognized by the specific anti-tag antibody for each tag. Twelve images per animal (n = 3) were counted per epitope tag. Results are expressed as means with standard error of the mean.
Lobbestael et al. BMC Biotechnology 2010 10:16 doi:10.1186/1472-6750-10-16