Table 2

Suspensions and extracts applied to patch test wells

Test material or species

Patch series type

Strain

Source

Cyanotoxin (concentration in 0.25% w/v lyophilised cyanobacteria patch preparation)


Sodium dodecyl sulfate (SDS, aka sodium lauryl sulfate)

Positive irritant control

Sigma-Aldrich P/L

Cylindrospermopsis raciborskii

Cyanobacterial cell suspension

AWT 205 Non-axenic

Australian Water Technologies culture collection Sydney, Australia

Cylindrospermopsin (2.0 mg/L)

C. raciborskii

Cyanobacterial LPS extract

AWT 205 Non-axenic

Australian Water Technologies culture collection Sydney, Australia

Microcystis aeruginosa

C. raciborskii

Aphanizomenon sp.

Cyanobacterial cell suspension

Field sample, North Pine Dam (South-east Queensland, Australia)

Microcystins (200 μg/L total microcystins expressed as MC-LR); cylindrospermopsin (6.4 μg/L)

M. aeruginosa

Cyanobacterial cell suspension

Field sample, Lake Coolmunda (Southern Queensland, Australia)

Non-toxic (nil detect for microcystins)

M. aeruginosa

Cyanobacterial LPS extract

Field sample, Lake Coolmunda (Southern Queensland, Australia)

M. aeruginosa

Cyanobacterial cell suspension

QH/NR/Ma/03 Non-axenic

Queensland Health Scientific Services culture collection, Brisbane, Australia

Microcystins [predominantly microcystin-LR] (1.60 mg/L total microcystins expressed as MC-LR)

Anabaena circinalis

Cyanobacterial cell suspension

Field sample, Lake Coolmunda (Southern Queensland, Australia)

Saxitoxins (19 μg/L total saxitoxins expressed as saxitoxin)

Planktothrix sp.

Cyanobacterial cell suspension

QH/NR/Px/01 Non-axenic

Queensland Health Scientific Services culture collection, Brisbane, Australia

Non-toxic (nil detect for microcystins)

Chlorella vulgaris

Green algal cell suspension

CS-42 Non-axenic

CSIRO collection of living microalgae, Hobart, Australia


Stewart et al. BMC Dermatology 2006 6:6   doi:10.1186/1471-5945-6-6

Open Data