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Mouse ear swelling tests (MESTs): positive control + three cyanobacteria suspensions + purified cylindrospermopsin |
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| TEST MATERIAL |
Proportion of test mice with ear swelling >20% after challenge |
Proportion of test mice with ear swelling >20% after re-challenge |
Per cent ear swelling of test group after challenge. Median (range) |
Per cent ear swelling of test group after re-challenge. Median (range) |
Per cent ear swelling of highest responder control mouse after challenge and re-challenge |
Fisher's exact test comparing proportion of ear thickness increases <20% between test and control mice |
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| 0.1% DNCB |
100% n= 10 at 24 hrs n = 9 at 24 & 48 hrs |
Not done |
68.6 (16.4 – 130.0) |
Not done |
Not done |
|
| 2% w/v M. aeruginosa microcystins 13.6 μg/mL |
0 |
0 |
1.9 (-11.6 – 10.7) |
1.2 (-1.6 – 10.0) |
8.9 |
|
| 2% w/v A. circinalis saxitoxins 6.0 μg/mL |
0 |
0 |
0.0 (-6.8 – 8.2) |
1.5 (-2.9 – 9.5) |
11.7 |
|
| 2% w/v C. raciborskii cylindrospermopsin 73 μg/mL |
60% n= 2 at 24 hrs n= 4 at 48 hrs |
60% n = 6 at 24 hrs n = 2 at 24 & 48 hrs |
15.8 (0.0 – 38.9) |
18.3 (2.2 – 47.5) |
10.7 |
p = 0.001 |
| 100 μg/mL cylindrospermopsin* |
22% n = 1 at 24 hrs n = 1 at 48 hrs |
44% n = 1 at 24 hrs n = 3 at 48 hrs |
8.1 (-4.8 – 26.1) |
12.6 (4.5 – 49.2) |
4.8 (challenge) 27.8 (re-challenge) |
p = 0.51 (challenge) p = 0.58 (rechallenge) |
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* challenge concentration applied to test ears was 50 μg/mL. Rechallenge concentration was 100 μg/mL (= induction concentration). | ||||||
Stewart et al. BMC Dermatology 2006 6:5 doi:10.1186/1471-5945-6-5 |
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