Early inflammatory markers in elicitation of allergic contact dermatitis
- Equal contributors
1 Dpto. Bioquímica Clínica, Facultad de Ciencias Químicas – Universidad Nacional de Córdoba, Córdoba, Argentina
2 Servicio de Alergia e Inmunología, Hospital Privado. Córdoba, Argentina
3 Patología, Facultad de Ciencias Químicas – Universidad Nacional de Córdoba, Córdoba, Argentina
4 Servicio de Dermatología, Hospital Privado, Córdoba, Argentina
5 Patología, Hospital Privado, Córdoba, Argentina
6 Universidad Nacional de Misiones (UNaM), Argentina
BMC Dermatology 2002, 2:9 doi:10.1186/1471-5945-2-9Published: 7 August 2002
Allergic Contact Dermatitis (ACD) is regarded as a T-cell-mediated delayed-type hypersensitivity reaction. We studied the kinetics of the expression of CS-1 fibronectin, thymus and activation-regulated chemokine (CCL17/ TARC) and different chemokine receptors (CR) in skin biopsies from individuals suffering from back problems, with the antigen responsible of their contact dermatitis and an irrelevant antigen.
Samples were taken at 2, 10, and 48 hours for histological and immunohistochemical studies using monoclonal antibodies against human CS-1 fibronectin, CCL17, CD3, CD68, CD49d, CXCR3, CCR5, and CCR3.
At positive antigen stimulated sites there was an early expression of CS-1 fibronectin (2 hours), followed by CCL17 and a later accumulation of alplha4/beta1+ (CD49d), CD3+, CD68+, CXCR3+ and CCR5+ mononuclear cells. At 48 hours, approximately 59 % of infiltrating cells were CXCR3+, 42% CCR5+, and only 14 % CCR3+.
These results showed for the first time a very early expression of CS-1 fibronectin which preceded production of CCL17 in blood endothelial cells (BCEs) from patients' skin with ACD. The role of these molecules in recruitment of monocytes and effector T cells in ACD is discussed.