Email updates

Keep up to date with the latest news and content from BMC Urology and BioMed Central.

Open Access Research article

The effect of indomethacin on the muscarinic induced contractions in the isolated normal guinea pig urinary bladder

Mohammad S Rahnama’i12*, Gommert A van Koeveringe12, Philip EV van Kerrebroeck12 and Stefan GG de Wachter1

Author Affiliations

1 Department of Urology, Maastricht University Medical Centre, PO Box 5800, Maastricht, 6202, AZ, The Netherlands

2 European Graduate School of Neuroscience, The Department of Psychiatry and Neuro psychology, Maastricht University, PO Box 616, Maastricht, 6200, MD, The Netherlands

For all author emails, please log on.

BMC Urology 2013, 13:8  doi:10.1186/1471-2490-13-8

Published: 7 February 2013

Abstract

Background

To investigate the effect of prostaglandin depletion by means of COX-inhibition on cholinergic enhanced spontaneous contractions.

Methods

The urethra and bladder of 9 male guinea pigs (weight 270–300 g) were removed and placed in an organ bath with Krebs’ solution. A catheter was passed through the urethra through which the intravesical pressure was measured. The muscarinic agonist arecaidine, the non-selective COX inhibitor indomethacin, and PGE2 were subsequently added to the organ bath. The initial average frequency and amplitude of spontaneous contractions in the first 2 minutes after arecaidine application were labelled Fini and Pini, respectively. The steady state frequency (Fsteady) and amplitude (Psteady) were defined as the average frequency and amplitude during the 5 minutes before the next wash out.

Results

Application of 1 μM PGE2 increased the amplitude of spontaneous contractions without affecting frequency. 10 μM of indomethacin reduced amplitude but not frequency.

The addition of indomethacin did not alter Fini after the first application (p = 0.7665). However, after the second wash, Fini was decreased (p = 0.0005). Fsteady, Psteady and Pini were not significantly different in any of the conditions. These effects of indomethacin were reversible by PGE2 addition..

Conclusions

Blocking PG synthesis decreased the cholinergically stimulated autonomous contractions in the isolated bladder. This suggests that PG could modify normal cholinergically evoked response. A combination of drugs inhibiting muscarinic receptors and PG function or production can then become an interesting focus of research on a treatment for overactive bladder syndrome.