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Open Access Research article

Altered expression of caveolin 2 and 3 in smooth muscle of rat urinary bladder by 17β-estradiol

Sun-Ouck Kim1, Seung Hee Song1, Seung-Chul Lee2, Kyung Aa Cho3, Jong Sung Park4, Dongdeuk Kwon1* and Kwangsung Park1

Author Affiliations

1 Department of Urology, Research Institute of Medical Sciences, Chonnam National University, 8, Hak-dong, Dong-ku, Gwangju 501-757, Korea

2 Department of Dermatology, Research Institute of Medical Sciences, Chonnam National University, Gwangju, Korea

3 Department of Biochemistry, Research Institute of Medical Sciences, Chonnam National University, Gwangju, Korea

4 Department of Physiology, Research Institute of Medical Sciences, Chonnam National University, Gwangju, Korea

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BMC Urology 2013, 13:44  doi:10.1186/1471-2490-13-44

Published: 16 September 2013

Abstract

Background

The purpose of this study was to investigate the effect of estrogen alteration on the expression of caveolin 2 and 3 in rat smooth muscle of urinary bladder.

Methods

Female Sprague–Dawley rats were divided into three groups: control, bilateral ovariectomy (Ovx), and bilateral ovariectomy followed by subcutaneous injections of 17β-estradiol (Ovx?+?Est). After 4 weeks, urodynamic measurements were taken to ascertain the contraction interval and contraction pressure. The expression and cellular localization of caveolin 2 and 3 were determined by Western blot and immunohistochemistry in rat urinary bladder smooth muscle.

Results

In cystometrograms, the contraction interval (min) was significantly lower in the Ovx group (3.1?±?1.5) than in the control group (5.6?±?1.2), but was increased after estrogen treatment (9.3?±?1.0). Conversely, the average contraction pressure (mmHg) was higher in the Ovx group (26.2?±?2.3) than in the control group (21.9?±?3.1), and was decreased after estrogen treatment (23.8?±?3.5). Caveolin 2 and 3 expression was localized in the cell membrane of the smooth muscle. The protein expression of both caveolin 2 and 3 was significantly lower after ovariectomy and was restored to the control levels after 17β-estradiol treatment.

Conclusions

Hormonal alteration causes a significant change in the expression of caveolin 2 and 3 in smooth muscle of rat urinary bladder. These findings suggest that these molecules might have functional roles in the detrusor overactivity that occurs in association with hormonal alteration.

Keywords:
Caveolin; Bladder; Estrogen; Rat