Induction of p21 is partially dependent on mTOR. (A) UMUC-3 cells were serum starved for 24 hours and then stimulated with 10 ng/ml EGF in the presence or absence of 1 hour pretreatment with 12.5 nM rapamycin. S6 phosphorylation at serines 235/236 was also measured to verify rapamycin-mediated inhibition of mTOR. (B) Quantitation of relative p21 induction by EGF in the presence of absence of rapamycin in UMUC- and UMUC-14 cells. Error bars represent S.E.M.
Yohn et al. BMC Urology 2011 11:19 doi:10.1186/1471-2490-11-19