Technical advance

An animal model in sheep for biocompatibility testing of biomaterials in cancellous bones

Katja MR Nuss¹ , Joerg A Auer¹ , Alois Boos² and Brigitte von Rechenberg¹

¹  Musculoskeletal Research Unit (MSRU), Equine Hospital, Vetsuisse Faculty, Zurich, Switzerland

²  Institute of Veterinary Anatomy, Vetsuisse Faculty, Zurich, Switzerland

author email corresponding author email

BMC Musculoskeletal Disorders 2006, 7:67doi:10.1186/1471-2474-7-67

Published:	15 August 2006

Abstract

Background

The past years have seen the development of many synthetic bone replacements. To test their biocompatibility and ability for osseointegration, osseoinduction and -conduction requires their placement within bone preferably in an animal experiment of a higher species.

Methods

A suitable experimental animal model in sheep with drill holes of 8 mm diameter and 13 mm depth within the proximal and distal humerus and femur for testing biocompatibility issues is introduced.

Results

This present sheep model allows the placing of up to 8 different test materials within one animal and because of the standardization of the bone defect, routine evaluation by means of histomorphometry is easily conducted. This method was used successfully in 66 White Alpine Sheep. When the drill holes were correctly placed no complications such as spontaneous fractures were encountered.

Conclusion

This experimental animal model serves an excellent basis for testing the biocompatibility of novel biomaterials to be used as bone replacement or new bone formation enhancing materials.