PCR analyses concerning influences of hyaluronic acid (HA) to the induction effect of PAMPS and PDMAAm gels on chondrogenic differentiation of ATDC5 cells. Each bar shows the mean and the standard deviation from 6 samples. Concerning the PAMPS gel, supplementation with HA of 0.01 and 0.10 mg/mL significantly increased expression of type-2 collagen gene (A) and aggrecan gene (B) than that without HA (“0 mg/mL“ shown in the photograph), while supplementation with HA of 1.00 mg/mL did not significantly affect expression of these genes. Regarding PDMAAm, supplementation with HA of 0.01 and 0.10 mg/mL did not significantly affect expression of type-2 collagen (C) and aggrecan genes (D), while supplementation with HA of 1.00 mg/mL significantly reduced expression of these genes in comparison with the culture without HA.
Yoshikawa et al. BMC Musculoskeletal Disorders 2013 14:56 doi:10.1186/1471-2474-14-56