Immunohistochemical and double immunofluorescence staining of lymphocyte aggregates. NRP1 was abundantly expressed by inflammatory cells of lymphocytes aggregates (A). Immunohistochemical staining of sequential sections showed that NRP1-positive cells in lymphocytes aggregates also expressed CD3 (B) or CD20 (C). Micrographs show the double immunofluorescence detection of NRP1 (D, green) and CD20 (E, red) with the merged images (F). The NRP1 positive signal colocalized with the CD20 positive signal in the inflammatory cells of lymphocyte aggregates. A–C, scale bars = 50 μm; D–F, scale bars = 10 μm.
Takagawa et al. BMC Musculoskeletal Disorders 2013 14:40 doi:10.1186/1471-2474-14-40