Immunofluorescence micrographs of stained myoblasts and 5-day myotube cultures. Staining with monoclonal antibodies recognizing titin epitopes in the Z-disk (A and E), A/I junction (B and F), A-band (C and G) and M-band (D and H) reveal development of mature cross-striated myofibrils. Nuclei were stained with DAPI (blue). The bars represent 10 μm.
Abdul-Hussein et al. BMC Musculoskeletal Disorders 2012 13:262 doi:10.1186/1471-2474-13-262