Research article

Assessing airway inflammation in clinical practice – experience with spontaneous sputum analysis

Olaf Holz¹ , Tanja Seiler¹ , Andreas Karmeier² , Jan Fraedrich³ , Helmut Leiner⁴ , Helgo Magnussen¹ , Rudolf A Jörres^1,5 and Lutz Welker¹

¹Hospital Großhansdorf, Center for Pneumology and Thoracic Surgery, 22927 Großhansdorf, Germany

²Outpatient clinic for internal medicine and pneumology, 20354 Hamburg, Germany

³Outpatient clinic for internal medicine and pneumology, 21029 Hamburg, Germany

⁴Outpatient clinic for internal medicine and pneumology, 24148 Kiel, Germany

⁵Institute and Outpatient Clinic for Occupational and Environmental Medicine, Ludwig-Maximilians-University, Munich, Germany

author email corresponding author email

BMC Pulmonary Medicine 2008, 8:5doi:10.1186/1471-2466-8-5

Published:	28 February 2008

Abstract

Background

The assessment of airway inflammation for the diagnosis of asthma or COPD is still uncommon in pneumology-specialized general practices. In this respect, the measurement of exhaled nitric oxide (NO), as a fast and simple methodology, is increasingly used. The indirect assessment of airway inflammation, however, does have its limits and therefore there will always be a need for methods enabling a direct evaluation of airway inflammatory cell composition. Sampling of spontaneous sputum is a well-known, simple, economic and non-invasive method to derive a qualitative cytology of airway cells and here we aimed to assess today's value of spontaneous sputum cytology in clinical practice.

Methods

Three pneumologists provided final diagnoses in 481 patients having sputum cytology and we retrospectively determined posterior versus prior probabilities of inflammatory airway disorders. Moreover, in a prospective part comprising 108 patients, pneumologists rated their confidence in a given diagnosis before and after knowing sputum cytology and rated its impact on the diagnostic process on an analogue scale.

Results

Among the 481 patients, 45% were diagnosed as having asthma and/or airway hyperresponsiveness. If patients showed sputum eosinophilia, the prevalence of this diagnosis was elevated to 73% (n = 109, p < 0.001). The diagnosis of COPD increased from 40 to 66% in patients with neutrophilia (n = 29, p < 0.01).

Thirty-three of the 108 patients were excluded from the prospective part (26 insufficient samples, 7 incomplete questionnaires). In 48/75 cases the confidence into a diagnosis was raised after knowing sputum cytology, and in 15/75 cases the diagnosis was changed as cytology provided new clues.

Conclusion

Our data suggest that spontaneous sputum cytology is capable of assisting in the diagnosis of inflammatory airway diseases in the outpatient setting. Despite the limitations by the semiquantitative assessment and lower sputum quality, the supportive power and the low economic effort needed can justify the use of this method, particularly if the diagnosis in question is thought to have an allergic background.