Table 3

Primers and PCR cycling conditions.

Primers

Sequences

Denaturation

annealing

Cycles

PCR products

HGF: Forward

Reverse

5'-CAGAGGGACAAAGGAAAAGAA-3'

5'-GCAAGTGAATGGAAGTCCTTTA-3'

94°C, 15s

58°C, 60s

40

167 bp

KGF: Forward

Reverse

5'-GAACAAGGAAGGAAAACTCTATGCAA-3'

5'-AAGTGGGCTGTTTTTTGTTCTTTCT-3'

94°C, 15s

60°C, 60s

40

201 bp

HGF-R: Forward

Reverse

5'-GTTTACTTGTTGCAAGGGAGAAGACT-3'

5'-TAGGGTGCCAGCATTTTAGCA-3'

94°C, 15s

58°C, 60s

40

88 bp

KGF-R: Forward

Reverse

5'-TTAAGCAGGAGCATCGCATTG-3'

5'-AACATCCAGGTGGTACGTGTGAT-3'

94°C, 15s

60°C, 60s

40

151 bp

Ubiquitin-c: Forward

Reverse

5'-CACTTGGTCCTGCGCTTGA-3'

5'-TTTTTTGGGAATGCAACAACTTT-3'

94°C, 15s

60°C, 60s

40

105 bp

Bonay et al. BMC Pulmonary Medicine 2005 5:13   doi:10.1186/1471-2466-5-13