|
Primers and PCR cycling conditions. |
|||||
| Primers |
Sequences |
Denaturation |
annealing |
Cycles |
PCR products |
|
|
|||||
| HGF: Forward Reverse |
5'-CAGAGGGACAAAGGAAAAGAA-3' 5'-GCAAGTGAATGGAAGTCCTTTA-3' |
94°C, 15s |
58°C, 60s |
40 |
167 bp |
| KGF: Forward Reverse |
5'-GAACAAGGAAGGAAAACTCTATGCAA-3' 5'-AAGTGGGCTGTTTTTTGTTCTTTCT-3' |
94°C, 15s |
60°C, 60s |
40 |
201 bp |
| HGF-R: Forward Reverse |
5'-GTTTACTTGTTGCAAGGGAGAAGACT-3' 5'-TAGGGTGCCAGCATTTTAGCA-3' |
94°C, 15s |
58°C, 60s |
40 |
88 bp |
| KGF-R: Forward Reverse |
5'-TTAAGCAGGAGCATCGCATTG-3' 5'-AACATCCAGGTGGTACGTGTGAT-3' |
94°C, 15s |
60°C, 60s |
40 |
151 bp |
| Ubiquitin-c: Forward Reverse |
5'-CACTTGGTCCTGCGCTTGA-3' 5'-TTTTTTGGGAATGCAACAACTTT-3' |
94°C, 15s |
60°C, 60s |
40 |
105 bp |
Bonay et al. BMC Pulmonary Medicine 2005 5:13 doi:10.1186/1471-2466-5-13 |
|||||