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Open Access Research article

Storage conditions for stability of offline measurement of fractional exhaled nitric oxide after collection for epidemiologic research

Yoshiko Yoda1, Naruhito Otani1, Hideki Hasunuma12, Hiroshi Kanegae2 and Masayuki Shima1*

Author Affiliations

1 Department of Public Health, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya, Hyogo 663-8501, Japan

2 Center for Environmental Information Science, 8-19 Yonban-cho, Chiyoda-ku, Tokyo, 102-0081, Japan

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BMC Pulmonary Medicine 2012, 12:68  doi:10.1186/1471-2466-12-68

Published: 2 November 2012

Additional files

Additional file 1:

In order to determine whether the different rates of change in NO concentration in the bags stored at 4°C related to smoking category are explained by the differences in initial concentration, the subjects were limited to non-smokers and ex-smokers whose initial FeNO levels were similar to those among current smokers (< 30ppb). In this case, FeNO levels among non-smokers (n = 14) in the bags stored at 4°C were 98.4% ± 5.3% and 113.8% ± 9.1% relative to the baseline values at 9 and 168 hours, respectively, and the values among ex-smokers (n = 4) were 97.0% ± 2.0% and 105.1% ± 7.4%, respectively. These values were smaller than those among current smokers (n = 6). However, the differences were not significant, because of the small number of subjects.

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