Table 1

Detection of Experimental Rhinovirus 16 Aerosols by Semi-nested RT-PCR of Filter Samples

Nebulizing Solution


Run Number

TCID50 per ml

Total ml Nebulized

Fractional Sampling Time per Filtera

Yieldb

Predicted TCID50 per Filterc

Positive Filters/Total


VA Run 12

16.1

15.2

0.136

0.041

1.4

3/3

VA Run 17

10.1

24.2

0.085

0.103

2.1

3/3

VA Run 13

6.5

15.0

0.142

0.093

1.3

2/3

VA Run 14

3.3

16.7

0.119

0.072

0.5

0/3


aSampling time of each filter (10 minutes) divided by total run time. bFluorescence yield (observed/expected) for sampling run. cPredicted = TCID50 per ml * Total ml nebulized * Fractional sampling time per filter * Yield.

Myatt et al. BMC Public Health 2003 3:5   doi:10.1186/1471-2458-3-5

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