Expression of GR mRNA in the OFC in controls, bipolar disorder cases and schizophrenia cases. Expression of GR mRNA in the OFC of controls (green diamonds), bipolar disorder cases (orange diamonds) and schizophrenia cases (blue diamonds). A) A trend towards an effect of diagnosis on pan GR in the OFC was observed (ANCOVA, p = 0.07), but no significant differences were seen in planned post-hoc tests comparing controls to schizophrenia or to bipolar disorder cases; B) There were subtle diagnostic differences in GR-1B mRNA expression in the OFC, with a 14.3% decrease in GR-1B expression in bipolar disorder cases relative to controls (p < 0.05) and a non-significant decrease in schizophrenia cases relative to controls (10.8%, p = 0.079); C and D) No significant diagnostic differences in GR-1C or GR-1F mRNA expression in the OFC were observed; E) GR-1H mRNA expression in the OFC was significantly decreased in schizophrenia cases relative to controls (22.0% decrease, p < 0.005) and in schizophrenia cases relative to bipolar disorder cases (19.5% decrease, p < 0.05); F) GR-1B, GR-1C, GR-1F and GR-1H mRNA transcript variants represented approximately 31.6%, 65.6%, 1.6% and 1.2% of total measured GR mRNA in the OFC respectively; G) Suicide-negative bipolar disorder (BP non-suicide) cases displayed 24.6% lower GR-1F mRNA than controls (p < 0.05), and 25.6% lower than suicide-positive bipolar disorder (BP suicide) cases (p < 0.05); H) Suicide-negative schizophrenia (SCZ non-suicide) cases displayed 26.5% lower GR-1H mRNA than controls (p < 0.001), and 27.9% lower than suicide-positive schizophrenia (SCZ suicide) cases (p < 0.05). I) There were no diagnostic differences in raw mRNA expression levels of housekeeping genes TBP, UBC, ACTB or B2M individually, nor the geomean of all four. Abbreviations: BP- bipolar disorder, SCZ- schizophrenia, CON- control. * p < 0.05, ** p < 0.005.
Sinclair et al. BMC Psychiatry 2012 12:84 doi:10.1186/1471-244X-12-84