Table 3

Comparison of broad range 16 S rDNA and conventional Bactec blood culture

Number of patients

Broad Range 16 S rDNA PCR


Sepsis

Negative

Positive


Bactec +

6#

2*

4


Bactec -

25

20

5


Not sepsis


Bactec +

0

0

0


Bactec -

17

16

1


# Culture from one patient grew four organisms (Klebsiella spp, Escherichia coli, a viridans Streptococcus and Staphylococcus aureus), culture from another patient grew two organisms (an unnamed viridans Streptococcus and Streptococcus gordomitis). Both had a positive PCR-result and were diagnosed with sepsis based on additional clinical and laboratory findings.

* One of these patients had an inconclusive PCR.

Reier-Nilsen et al. BMC Pediatrics 2009 9:5   doi:10.1186/1471-2431-9-5

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