Figure 5.

Expression and localization of Palm in chick retinal cultures. Cultures were immunostained with polyclonal anti-Palm (A, D) and RT-97 anti-neurofilament (B, E) antibodies after 2 (A-C) or 7 (D-F) days in culture. For each pair, the merged images are shown in C and F. After 2 days in culture, Palm is present on most cells at cell borders as well as intracellular puncta (A). Fine processes resembling axons (arrows) that are sometimes positive for RT-97 (B) are also labeled. After 7 days in culture, Palm staining appears punctate but more diffuse (D), and does not appear to be localized on the numerous long processes stained with RT-97 (F). Bar in D, 25μm. Green- Palm; Red-RT-97;

Castellini et al. BMC Ophthalmology 2005 5:14   doi:10.1186/1471-2415-5-14
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