Table 3 |
|||||
|
Transcript expression by qRT-PCR in relation to protein expression and gene amplification using IHC and CISH/FISH methodologies, respectively. |
|||||
|
qRT-PCR |
|||||
|
|
|||||
|
n (%) |
HER-2 ratio (range) * |
P |
Ratio ≤ 2.00 n (%) |
Ratio >2.00 n (%) |
|
|
|
|||||
|
IHC |
|||||
|
0 or 1+ |
50 (66.7) |
0.93 (0.05–12.24) |
<0.0001a |
40 (80.0) |
10 (20.0) |
|
2+ |
12 (17.3) |
2.46 (1.15–7.61) |
5 (41.7) |
7 (58.3) |
|
|
3+ |
13 (16.0) |
7.55 (1.07–20.43) |
1 (7.7) |
12 (92.3) |
|
|
Total |
75 |
||||
|
CISH |
|||||
|
No amplification |
24 (64.9) |
0.75 (0.05–1.95) |
<0.0001b |
24 (100) |
0 (0.0) |
|
High amplification |
13 (35.1) |
4.69 (1.07–20.43) |
2 (15.4) |
11 (84.6) |
|
|
Total |
37 |
||||
|
FISH |
|||||
|
No amplification |
0 (0.0) |
0 (0.0) |
ND |
0 (0.0) |
0 (0.0) |
|
Amplification |
8 (100) |
7.93 (2.64–20.43) |
0 (0.0) |
8 (100) |
|
|
Total |
8 |
||||
|
|
|||||
|
* Mean HER-2/reference gene ratios; ND: not determined. a Kruskal-Wallis test. b Mann-Whitney test. |
|||||
|
Rosa et al. BMC Cancer 2009 9:90 doi:10.1186/1471-2407-9-90 |
|||||
